Kalisch and colleagues developed one of the reliable and valid measures of missed nursing care, the MISSCARE survey, which has been used extensively in medical surgical care and has been adapted to pediatric and neonatal intensive care. Our team previously adapted the MISSCARE Survey for the labor and birth portion of perinatal care. The resulting Perinatal Missed Care Survey incorporates nursing care and critical surveillance activities specific to labor and birth. We recently demonstrated Perinatal Missed Care Survey acceptability, reliability, and a factor structure consistent with the original instrument. The purpose of the present study was to confirm reliability and validity of the Perinatal Missed Care Survey in a large sample of nurses and hospitals, test construct validity with confirmatory factor analysis, and describe the prevalence of missed nursing care during labor and birth.